Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2021-07-29 |
タイトル |
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タイトル |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
タイトル |
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言語 |
en |
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タイトル |
Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite |
言語 |
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言語 |
eng |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Antifreeze protein |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Arctic bacteria |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Cryoconite |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Glacier |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
nanoLC-ESI-MS/MS |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
著者 |
Singh, Purnima
Tsuda, Sakae
Singh, Shiv Mohan
Mondal, Sukanta
Roy, Utpal
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抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Antifreeze proteins (AFPs) play important roles in the survival of microorganisms existing in the cold environment. One of the several AFPs from an Arctic glacier bacterial strain which in our previous study showed thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activities has been characterized partially in the present study. The cry-c AFP from the Svalbard Arctic cryoconite strain Cry-c MLB-29 belonging to the genus Cryobacterium has been characterized by ESI-Q-TOF-MS that revealed its molecular mass as 22.14 kDa. Multi-enzymatic limited digestion and nano-LC-ESI-MS/MS analyses revealed 228 amino acid residues in 21 peptide fragments. First 20 amino acid residues of the N-terminus of cry-c AFP has been deduced. Glycine and alalnine-rich sequences and the NXS motifs in several peptide fragments have been found out. Selected peptide fragments of the AFP have been modeled by PEP-FOLD and the structure-function correlation discussed in view of its antifreeze property. |
書誌情報 |
en : Polar Science
巻 28,
p. 100661,
発行日 2021-06
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ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
18739652 |
DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.polar.2021.100661 |
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関連名称 |
10.1016/j.polar.2021.100661 |