{"created":"2023-07-25T11:11:48.817164+00:00","id":16496,"links":{},"metadata":{"_buckets":{"deposit":"c838a217-030e-431d-8d6a-1d6c31a3315f"},"_deposit":{"created_by":26,"id":"16496","owners":[26],"pid":{"revision_id":0,"type":"depid","value":"16496"},"status":"published"},"_oai":{"id":"oai:nipr.repo.nii.ac.jp:00016496","sets":["1259:1267:2053"]},"author_link":["72569","72572","72573","72571","72570"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"100661","bibliographicVolumeNumber":"28","bibliographic_titles":[{},{"bibliographic_title":"Polar Science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Antifreeze proteins (AFPs) play important roles in the survival of microorganisms existing in the cold environment. One of the several AFPs from an Arctic glacier bacterial strain which in our previous study showed thermal hysteresis (TH) and ice recrystallization inhibition (IRI) activities has been characterized partially in the present study. The cry-c AFP from the Svalbard Arctic cryoconite strain Cry-c MLB-29 belonging to the genus Cryobacterium has been characterized by ESI-Q-TOF-MS that revealed its molecular mass as 22.14 kDa. Multi-enzymatic limited digestion and nano-LC-ESI-MS/MS analyses revealed 228 amino acid residues in 21 peptide fragments. First 20 amino acid residues of the N-terminus of cry-c AFP has been deduced. Glycine and alalnine-rich sequences and the NXS motifs in several peptide fragments have been found out. Selected peptide fragments of the AFP have been modeled by PEP-FOLD and the structure-function correlation discussed in view of its antifreeze property.","subitem_description_type":"Abstract"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.polar.2021.100661"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.polar.2021.100661","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18739652","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Singh, Purnima","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuda, Sakae","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Singh, Shiv Mohan","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mondal, Sukanta","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Roy, Utpal","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Antifreeze protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Arctic bacteria","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryoconite","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glacier","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nanoLC-ESI-MS/MS","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite"},{"subitem_title":"Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"26","path":["2053"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-07-29"},"publish_date":"2021-07-29","publish_status":"0","recid":"16496","relation_version_is_last":true,"title":["Partial characterization of an antifreeze protein (CRY-c) from Cryobacterium psychrotolerans MLB-29 of Arctic glacier cryoconite"],"weko_creator_id":"26","weko_shared_id":-1},"updated":"2023-07-25T12:51:53.871604+00:00"}