Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
公開日 |
1989-09-01 |
タイトル |
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タイトル |
Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
ID登録 |
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ID登録 |
10.15094/00005060 |
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ID登録タイプ |
JaLC |
ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
著者名(英) |
Asakawa, Makio
Nakagawa, Hiroki
Fukuda, Yasushi
Fukuchi, Mitsuo
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著者所属(英) |
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en |
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Faculty of Education, Kumamoto University |
著者所属(英) |
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en |
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Department of Agricultural Chemistry,Faculty of Agriculture, Saga University |
著者所属(英) |
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en |
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Faculty of Education, Kumamoto University |
著者所属(英) |
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en |
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National Institute of Polar Research |
抄録(英) |
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内容記述タイプ |
Other |
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内容記述 |
The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum. |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10819561 |
書誌情報 |
Proceedings of the NIPR Symposium on Polar Biology
巻 2,
p. 131-138,
発行日 1989-09
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出版者 |
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出版者 |
National Institute of Polar Research |