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Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
https://doi.org/10.15094/00005060
https://doi.org/10.15094/0000506081406cd2-0bdd-4ae1-8243-f92e556383e4
名前 / ファイル | ライセンス | アクション |
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KJ00000036828 (1.3 MB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 1989-09-01 | |||||
タイトル | ||||||
タイトル | Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15094/00005060 | |||||
ID登録タイプ | JaLC | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
著者名(英) |
Asakawa, Makio
× Asakawa, Makio× Nakagawa, Hiroki× Fukuda, Yasushi× Fukuchi, Mitsuo |
|||||
著者所属(英) | ||||||
en | ||||||
Faculty of Education, Kumamoto University | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Agricultural Chemistry,Faculty of Agriculture, Saga University | ||||||
著者所属(英) | ||||||
en | ||||||
Faculty of Education, Kumamoto University | ||||||
著者所属(英) | ||||||
en | ||||||
National Institute of Polar Research | ||||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10819561 | |||||
書誌情報 |
Proceedings of the NIPR Symposium on Polar Biology 巻 2, p. 131-138, 発行日 1989-09 |
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出版者 | ||||||
出版者 | National Institute of Polar Research |