{"created":"2023-07-25T11:02:42.598221+00:00","id":5060,"links":{},"metadata":{"_buckets":{"deposit":"e4bcee39-92f2-49df-b177-2e973fbde3fc"},"_deposit":{"created_by":4,"id":"5060","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"5060"},"status":"published"},"_oai":{"id":"oai:nipr.repo.nii.ac.jp:00005060","sets":["1259:1260:1263:636:638"]},"author_link":["8408","1646","25285","1871"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-09","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"138","bibliographicPageStart":"131","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"Proceedings of the NIPR Symposium on Polar Biology"}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Asakawa, Makio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakagawa, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuda, Yasushi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukuchi, Mitsuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum.","subitem_description_type":"Other"}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15094/00005060","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_22":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Institute of Polar Research"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA10819561","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Faculty of Education, Kumamoto University"},{"subitem_text_language":"en","subitem_text_value":"Department of Agricultural Chemistry,Faculty of Agriculture, Saga University"},{"subitem_text_language":"en","subitem_text_value":"Faculty of Education, Kumamoto University"},{"subitem_text_language":"en","subitem_text_value":"National Institute of Polar Research"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1989-09-01"}],"displaytype":"detail","filename":"KJ00000036828.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"KJ00000036828","url":"https://nipr.repo.nii.ac.jp/record/5060/files/KJ00000036828.pdf"},"version_id":"1cb47ee5-c38d-459b-be3a-15ae8451f82b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii"}]},"item_type_id":"1","owner":"4","path":["638"],"pubdate":{"attribute_name":"公開日","attribute_value":"1989-09-01"},"publish_date":"1989-09-01","publish_status":"0","recid":"5060","relation_version_is_last":true,"title":["Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-07-25T12:14:39.255885+00:00"}