@article{oai:nipr.repo.nii.ac.jp:00005060,
 author = {Asakawa, Makio and Nakagawa, Hiroki and Fukuda, Yasushi and Fukuchi, Mitsuo},
 journal = {Proceedings of the NIPR Symposium on Polar Biology},
 month = {Sep},
 note = {P(論文), The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum.},
 pages = {131--138},
 title = {Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii},
 volume = {2},
 year = {1989}
}