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A sequencing protocol of some DNA regions in nuclear, chloroplastic and mitochondrial genomes with an individual colony of Thalassiosira nordenskioeldii Cleve (Bacillariophyceae)
https://doi.org/10.15094/00006223
https://doi.org/10.15094/00006223bc00b892-ae17-4f61-8e59-8a9e85f0e60f
名前 / ファイル | ライセンス | アクション |
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KJ00002366632 (668.4 kB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2005-01-01 | |||||
タイトル | ||||||
タイトル | A sequencing protocol of some DNA regions in nuclear, chloroplastic and mitochondrial genomes with an individual colony of Thalassiosira nordenskioeldii Cleve (Bacillariophyceae) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Bacillariophyceae, chelating agent, DNA sequence, micromanipulation, Thallassiosira nordenskioeldii | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15094/00006223 | |||||
ID登録タイプ | JaLC | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
論文名よみ | ||||||
その他のタイトル | A sequencing protocol of some DNA regions in nuclear, chloroplastic and mitochondrial genomes with an individual colony of Thalassiosira nordenskioeldii Cleve (Bacillariophyceae) | |||||
著者名(英) |
Iwatani,Naohiro
× Iwatani,Naohiro× Murakami,Satoru× Suzuki,Yoshihiro |
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著者所属(英) | ||||||
言語 | en | |||||
値 | Department of Biological Science, School of Science, Kanagawa University/Department of Biological Science, School of Science, Kanagawa University/Department of Biological Science, School of Science, Kanagawa University | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | From cultured cells of micro algal species of Thalassiosira nordenskioeldii Cleve, total DNA was extracted with a buffer containing a chelating agent, Chelex 100. Each DNA fragment for 18s rDNA, 16s rDNA and coxI in nuclear, chloroplastic and mitochondrial genomes, respectively, were amplified from the DNA extracts by polymerase chain reactions, and their sequences were determined. This protocol for determining DNA sequences, was scaled down for the DNA extracts from small amounts of cells. Finally, DNA sequences in genomes were determined with 10 cells collected by a micromanipulation technique. The quantity of cells corresponded to an individual colony of this species. With this protocol, DNA sequences in various regions in genomes of each individual micro algal species living in natural environments can be determined directly, without isolation of strains and cultures. | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11327019 | |||||
書誌情報 |
Polar bioscience 巻 18, p. 35-45, 発行日 2005-01-01 |
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出版者 | ||||||
出版者 | National Institute of Polar Research |