{"_buckets": {"deposit": "d43e1efc-efc9-4814-a17a-26f7b86d49c8"}, "_deposit": {"created_by": 4, "id": "5346", "owners": [4], "pid": {"revision_id": 0, "type": "depid", "value": "5346"}, "status": "published"}, "_oai": {"id": "oai:nipr.repo.nii.ac.jp:00005346", "sets": ["646"]}, "author_link": ["9414", "9415", "9419", "9417", "9416", "9418"], "item_1_alternative_title_5": {"attribute_name": "論文名よみ", "attribute_value_mlt": [{"subitem_alternative_title": "IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology)"}]}, "item_1_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1997-02", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "162", "bibliographicPageStart": "153", "bibliographicVolumeNumber": "10", "bibliographic_titles": [{"bibliographic_title": "Proceedings of the NIPR Symposium on Polar Biology"}]}]}, "item_1_creator_7": {"attribute_name": "著者名よみ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "オクヤマ, ヒデトシ"}], "nameIdentifiers": [{"nameIdentifier": "9414", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "エナリ, ダイスケ"}], "nameIdentifiers": [{"nameIdentifier": "9415", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "モリタ, ナオキ"}], "nameIdentifiers": [{"nameIdentifier": "9416", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_8": {"attribute_name": "著者名(英)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OKUYAMA, Hidetoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9417", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ENARI, Daisuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9418", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MORITA, Naoki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9419", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "A cell-free extract of Pseudomonas sp. strain E-3 (Pseudomonas E-3) had activities that catalyzed the conversion of 9-cis-hexadecenoic acid [16:1(9c)] to 9-trans-hexadecenoic acid [16:1(9t)] in the free acid form, and when 16:1(9c) was esterified to phosphatidylethanolamine (PE). A soluble 16:1(9c) cis-trans isomerase (9-Iase) was purified to complete homogeneity from the extract of Pseudomonas E-3 and characterized. Electrophoresis on both denaturing and incompletely-denaturing polyacrylamide gels of the purified enzyme preparation showed the single band of a protein with a molecular mass of 80 kDa, suggesting that the 9-Iase is a monomeric protein of 80 kDa. The 9-Iase, assayed with 16:1(9c) as a substrate, had a specific activity of 22.8 μmol per h per mg of protein and a Km of 118 μM. The enzyme had the optimum temperature for catalysis at 30℃ and catalyzed the cis to trails conversion of a double bond of 16:1(9c) in the free acid form, but it was able to isomerize 16:1(9c) esterified to PE in the presence of the cell membrane fraction. Irrespective of the temperature at which cells of Pseudomonas E-3 were grown, the level of 16:1(9t) was around 2-4% of the total cellular fatty acids. However, when cells grown at 4℃ were warmed up to 30℃ at a rate of about 20℃/min, the level of 16:1(9t) was increased from 3% to 14%. Since the level in situ of free fatty acids in this bacterium is negligible, it is suggested that the 9-Iase is operative in vivo as the cis to trans isomerase of 16:1(9c) that is esterified to PE together with the membranous factor, and that the 9-Iase might work as a stringent modulator of membrane fluidity under abrupt alteration in growth temperature.", "subitem_description_type": "Other"}]}, "item_1_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.15094/00005346", "subitem_identifier_reg_type": "JaLC"}]}, "item_1_publisher_22": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "National Institute of Polar Research"}]}, "item_1_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10819561", "subitem_source_identifier_type": "NCID"}]}, "item_1_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University"}, {"subitem_text_language": "en", "subitem_text_value": "Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Biological Science, Graduate School of Science, Hokkaido University:(Present address)Laboratory of Biochemistry, Hokkaido National Industrial Research Institute, AIST"}]}, "item_1_text_3": {"attribute_name": "記事種別(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Proceeding"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "1997-02-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000767689.pdf", "filesize": [{"value": "877.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_6", "mimetype": "application/pdf", "size": 877400.0, "url": {"label": "KJ00000767689", "url": "https://nipr.repo.nii.ac.jp/record/5346/files/KJ00000767689.pdf"}, "version_id": "2b8b5b91-d11e-432c-a4f8-126049f32159"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology)", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology)"}]}, "item_type_id": "1", "owner": "4", "path": ["646"], "permalink_uri": "https://doi.org/10.15094/00005346", "pubdate": {"attribute_name": "公開日", "attribute_value": "1997-02-01"}, "publish_date": "1997-02-01", "publish_status": "0", "recid": "5346", "relation": {}, "relation_version_is_last": true, "title": ["IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology)"], "weko_shared_id": 4}