Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
公開日 |
2006-12-01 |
タイトル |
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タイトル |
A new application of the SFDA-staining method to assessment of the freezing tolerance in leaves of alpine plants |
言語 |
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言語 |
eng |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
alpine plants |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
FDA (fluorescein diacetate) |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
freezing tolerance |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
SFDA (5- (6-) sulfofluorescein diacetate) |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
survival ratio |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
ID登録 |
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ID登録 |
10.15094/00006262 |
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ID登録タイプ |
JaLC |
ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
著者名(英) |
Yamori, Wataru
Kogami, Hiroyuki
Yoshimura, Yoshitaka
Tsuji, Takashi
Masuzawa, Takehiro
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著者所属(英) |
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en |
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Department of Biology and Geosciences, Faculty of Science, Shizuoka University |
著者所属(英) |
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en |
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Department of Biology and Geosciences, Faculty of Science, Shizuoka University |
著者所属(英) |
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en |
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Faculty of Agriculture, Tamagawa University |
著者所属(英) |
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en |
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Research Institute, Tamagawa University |
著者所属(英) |
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en |
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Department of Biology and Geosciences, Faculty of Science, Shizuoka University |
抄録(英) |
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内容記述タイプ |
Other |
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内容記述 |
For the first time, this study used 5- (6-) sulfofluorescein diacetate (SFDA), a fluorescent product in plant cells converted by esterase activity to fluorescein-5- (and 6-) sulfonic acid (FSA), to assess the freezing tolerance of leaf cells. We were able to readily distinguish living and dead cells, and detect differences in freezing tolerance among five alpine plants using the SFDA-staining method. We also compared this method with two conventional methods, the electrolyte leakage test and fluorescein diacetate (FDA) staining method. The electrolyte leakage test often over- or underestimated freezing injury. With the uninjured control samples, the FDA-staining method failed to stain all leaf cells, while the SFDA-staining method stained almost 100%. From these results, we concluded that SFDA-staining is a more convenient, accurate and reproducible method for analyses of freezing tolerance. |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11327019 |
書誌情報 |
Polar bioscience
巻 20,
p. 82-91,
発行日 2006-12
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出版者 |
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出版者 |
National Institute of Polar Research |