{"created":"2023-07-25T11:03:23.335823+00:00","id":6262,"links":{},"metadata":{"_buckets":{"deposit":"1d8e3400-3aca-4ed7-8296-4abc27bf5e64"},"_deposit":{"created_by":4,"id":"6262","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"6262"},"status":"published"},"_oai":{"id":"oai:nipr.repo.nii.ac.jp:00006262","sets":["1259:1260:1263:792:801"]},"author_link":["18999","10095","19580","42922","42920"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"91","bibliographicPageStart":"82","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Polar bioscience"}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamori, Wataru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kogami, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimura, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuji, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masuzawa, Takehiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"For the first time, this study used 5- (6-) sulfofluorescein diacetate (SFDA), a fluorescent product in plant cells converted by esterase activity to fluorescein-5- (and 6-) sulfonic acid (FSA), to assess the freezing tolerance of leaf cells. We were able to readily distinguish living and dead cells, and detect differences in freezing tolerance among five alpine plants using the SFDA-staining method. We also compared this method with two conventional methods, the electrolyte leakage test and fluorescein diacetate (FDA) staining method. The electrolyte leakage test often over- or underestimated freezing injury. With the uninjured control samples, the FDA-staining method failed to stain all leaf cells, while the SFDA-staining method stained almost 100%. From these results, we concluded that SFDA-staining is a more convenient, accurate and reproducible method for analyses of freezing tolerance.","subitem_description_type":"Other"}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15094/00006262","subitem_identifier_reg_type":"JaLC"}]},"item_1_publisher_22":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Institute of Polar Research"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11327019","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Biology and Geosciences, Faculty of Science, Shizuoka University"},{"subitem_text_language":"en","subitem_text_value":"Department of Biology and Geosciences, Faculty of Science, Shizuoka University"},{"subitem_text_language":"en","subitem_text_value":"Faculty of Agriculture, Tamagawa University"},{"subitem_text_language":"en","subitem_text_value":"Research Institute, Tamagawa University"},{"subitem_text_language":"en","subitem_text_value":"Department of Biology and Geosciences, Faculty of Science, Shizuoka University"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2006-12-01"}],"displaytype":"detail","filename":"KJ00004531451.pdf","filesize":[{"value":"325.5 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"KJ00004531451","url":"https://nipr.repo.nii.ac.jp/record/6262/files/KJ00004531451.pdf"},"version_id":"59406b77-d5ab-44c4-9e65-49b394c9c3a0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"alpine plants","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FDA (fluorescein diacetate)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"freezing tolerance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SFDA (5- (6-) sulfofluorescein diacetate)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"survival ratio","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A new application of the SFDA-staining method to assessment of the freezing tolerance in leaves of alpine plants","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A new application of the SFDA-staining method to assessment of the freezing tolerance in leaves of alpine plants"}]},"item_type_id":"1","owner":"4","path":["801"],"pubdate":{"attribute_name":"公開日","attribute_value":"2006-12-01"},"publish_date":"2006-12-01","publish_status":"0","recid":"6262","relation_version_is_last":true,"title":["A new application of the SFDA-staining method to assessment of the freezing tolerance in leaves of alpine plants"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-07-25T12:03:51.138278+00:00"}