{"created":"2023-07-25T11:06:18.342807+00:00","id":9663,"links":{},"metadata":{"_buckets":{"deposit":"1ddf9802-f36a-4f5c-a6d6-f10e9a0cd6f4"},"_deposit":{"created_by":4,"id":"9663","owners":[4],"pid":{"revision_id":0,"type":"depid","value":"9663"},"status":"published"},"_oai":{"id":"oai:nipr.repo.nii.ac.jp:00009663","sets":["1259:906:1251:1167"]},"author_link":["17351","7808","17350","17334","17359","15978","17358","15976"],"item_1_alternative_title_5":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_alternative_title":"Percoll ミツド コウバイ ヲ モチイタ エンシンブンリ ニヨル イシクラゲ ノ サイボウガイタトウ (EPS) ジョキョ サイボウ ノ タンリホウ"}]},"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-11-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"293","bibliographicPageStart":"285","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"南極資料"},{"bibliographic_title":"Antarctic Record","bibliographic_titleLang":"en"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小杉, 真貴子"},{"creatorName":"コスギ, マキコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"菓子野, 康浩"},{"creatorName":"カシノ, ヤスヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"工藤, 栄"},{"creatorName":"クドウ, サカエ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伊村, 智"},{"creatorName":"イムラ, サトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kosugi, Makiko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kashino, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kudoh, Sakae ","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imura, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"陸生ラン色細菌のイシクラゲ(Nostoc commune)は南極や日本にも生育する汎存種である.イシクラゲの細胞の周りに存在する細胞外多糖(EPS)は,水分保持や細胞の保護に役立つと共に遮光性物質を蓄積しており,これが苛酷な環境への適応に重要な役割を果たしていると考えられている.南極産の貴重なイシクラゲを材料にしてこの仮説を検証するためには,EPSを除去した細胞を高収率で回収する必要がある.そこで,これまでに報告のあるEPS除去方法を改良し,Percollを用いた遠心分離で細胞の回収率向上を図った. その結果, イシクラゲ破砕懸濁液に10%濃度のPercollを混合して遠心することでEPSを効率良く除去できることが分かった.更に細胞画分を80% と50% のPercoll に上層して遠心することで他の不純物の除去が達成され,少量のイシクラゲを生理学実験に用いるのに適した細胞単離法が確立された.","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The terrestrial cyanobacterium Nostoc commune Vaucher ex Bornet et Flahault occurs worldwide, including in Japan and Antarctica. N. commune has a large amount of extracellular polysaccharides (EPS) that hold moisture and protect the cells and at the same time accumulate light-blocking substances which is believed to play an important part in adaptation to a severe environment. To evaluate the photoadaptation processes in N. commune and clarify the role(s) of EPS under ambient environmental condition at Antarctica, separation of cells from EPS is necessary. High yield is a prerequisite for the use of only small amount of natural N. commune from Antarctica. For this purpose, we developed a separation method by improving the Percoll density gradient centrifugation method using an EPS-coated field-grown Nostoc population. We established the most suitable condition to separate naked cells from EPS at high yield retaining high photosynthetic activity. The method is composed of centrifugation of cell homogenated N. commune in 10% (v/v) Percoll to separate cells efficiently from EPS followed by fractionating centrifugation to remove impurities using the gradient of Percoll (80% and 50%, v/v).","subitem_description_type":"Other"}]},"item_1_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15094/00009663","subitem_identifier_reg_type":"JaLC"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN00181831","subitem_source_identifier_type":"NCID"}]},"item_1_source_id_20":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00857289","subitem_source_identifier_type":"ISSN"}]},"item_1_subject_21":{"attribute_name":"国際十進分類法","attribute_value_mlt":[{"subitem_subject":"59(21)","subitem_subject_scheme":"UDC"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"National Institute of Polar Research, Research Organization of Information and Systems, Midori-cho 10-3, Tachikawa, Tokyo 190-8518."},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297."},{"subitem_text_language":"en","subitem_text_value":"National Institute of Polar Research, Research Organization of Information and Systems, Midori-cho 10-3, Tachikawa, Tokyo 190-8518."},{"subitem_text_language":"en","subitem_text_value":"National Institute of Polar Research, Research Organization of Information and Systems, Midori-cho 10-3, Tachikawa, Tokyo 190-8518."}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"情報・システム研究機構国立極地研究所"},{"subitem_text_value":"兵庫県立大学大学院生命理学研究科"},{"subitem_text_value":"情報・システム研究機構国立極地研究所"},{"subitem_text_value":"情報・システム研究機構国立極地研究所"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2012-11-30"}],"displaytype":"detail","filename":"KJ00008446719.pdf","filesize":[{"value":"466.7 kB"}],"format":"application/pdf","licensetype":"license_6","mimetype":"application/pdf","url":{"label":"KJ00008446719","url":"https://nipr.repo.nii.ac.jp/record/9663/files/KJ00008446719.pdf"},"version_id":"e33a9557-ee34-4636-bbb9-d4a824e6be97"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Percoll密度勾配を用いた遠心分離によるイシクラゲの細胞外多糖(EPS)除去細胞の単離法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Percoll密度勾配を用いた遠心分離によるイシクラゲの細胞外多糖(EPS)除去細胞の単離法"},{"subitem_title":"Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation","subitem_title_language":"en"}]},"item_type_id":"1","owner":"4","path":["1167"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-30"},"publish_date":"2012-11-30","publish_status":"0","recid":"9663","relation_version_is_last":true,"title":["Percoll密度勾配を用いた遠心分離によるイシクラゲの細胞外多糖(EPS)除去細胞の単離法"],"weko_creator_id":"4","weko_shared_id":4},"updated":"2023-07-25T14:10:09.805608+00:00"}