@article{oai:nipr.repo.nii.ac.jp:00009176, author = {大野, 義一朗 and 宮田, 敬博 and 吉田, 和隆 and 大谷, 眞二 and 草谷, 洋光 and 山本, 啓之 and Ohno, Giichiro and Miyata, Takahiro and Yoshida, Kazutaka and Ohtani, Shinji and Kusagaya, Hiromitsu and Yamamoto, Hiroyuki}, issue = {3}, journal = {南極資料, Antarctic Record}, month = {Mar}, note = {P(論文), 24時間風呂は微生物が繁殖しやすく, 越冬期間中の状況では診断治療が難しいレジオネラ症の感染源として危険性が指摘されている。JARE-39 (1998-1999), -40 (1999-2000)は昭和基地の24時間風呂の細菌調査を行った。浴槽水, 130kl造水槽水, 24時間風呂循環回路のフィルターを持ち帰りレジオネラ菌とその他の細菌について培養検査, PCR検査を行った。またJARE-40で越冬中に浴槽水の一般細菌と大腸菌の培養検査, 浴槽水温調査を行った。さらに疾患統計から風呂に起因する可能性のある疾患の発生を調査した。その結果フィルターと浴槽水からPCRにてLegionella pneumophilaと塩基配列が一致するレジオネラ菌遺伝子を検出したがレジオネラ菌分離培養は陰性であった。8月の浴槽水温は平均44.6℃と高温で少数の一般細菌が検出されたが, 水温が1-2℃低下した越冬後半には一般細菌が増加し大腸菌群も検出された。39次隊越冬中に胃腸炎と上気道炎の集団発生があったが風呂との因果関係は不明であった。今後も定期検査が望ましく, 遠隔地で可能な検査体制の確立が望まれる。, Background; The circulating bath is known to have the potential to allow growth of microorganisms including pathogenic bacteria such as Legionella. Legionellosis, which causes severe pneumonia, may be difficult to manage in the Antarctic winter season. JARE-39 (1998-1999) and -40 (1999-2000) did microbiological studies of Syowa Station's circulating bath. Methods; Water samples including bath sink, water supplying outer tank, and filter of bath circulation system were collected and stored by freezing or in cold storage. After the samples were brought back to Japan, they were expamined to detect colonies of legionellae and other microorganisms; DNA testing to detect legionellae by PCR was also carried out. JARE-40 determined total counts of bacteria and Escherichia coli in the bath, and periodically measured the water temperature. Epidemiological analysis of bath-related diseases was done for JARE-39. Findings; Specimens from bath-water and filter of water-supply system showed positive result of the Legionella-specific DNA by PCR method. These samples, however, were all negative by the culture method for legionellae. Bath-water temperature in August exceeded 44.6℃ on average. By the end of the winter the water temperature came down 1 to 2 degrees, and then total counts of bacteria and E. coli were measured. During the winter season of JARE-39,outbreaks of gastroenteritis and upper respiratory illness occurred. Systematic microbiological and epidemiological surveillance in the station is necessary to prevent outbreaks of infectious diseases.}, pages = {311--319}, title = {南極昭和基地における循環式風呂の微生物調査-レジオネラ菌と一般細菌について-}, volume = {45}, year = {2002}, yomi = {オオノ, ギイチロウ and ミヤタ, タカヒロ and ヨシダ, カズタカ and オオタニ, シンジ and クサガヤ, ヒロミツ and ヤマモト, ヒロユキ} }