@article{oai:nipr.repo.nii.ac.jp:00005369, author = {ホシノ, タモツ and / マツモト, ナオユキ and アラキ, タダシ and / ゴウダ, タカコ and オオギヤ, サトル and イシザキ, コウゾウ and HOSHINO, Tamotsu and TRONSMO, Anne Marte and MATSUMOTO, Naoyuki and ARAKI, Tadashi and GEORGES, Fawzy and GODA, Takako and OHGIYA, Satoru and ISHIZAKI, Kozo}, journal = {Proceedings of the NIPR Symposium on Polar Biology}, month = {Feb}, note = {P(論文), Frost tolerance was determined in Norwegian varieties of groups I and III of the psychrophihc fungus Typhula mhikanensis. When myceha of group I and III strains were rapidly frozen to -40℃ in a program freezer at a cooling rate of 20℃/h, the regrowth of group I strains was delayed at their optimal growth temperature (10℃), whereas the regrowth of group III strains was not affected at their optimal growth temperature (2℃). Furthermore, when group I and III strains were rapidly frozen and regrew at 0℃, the average temperature during snowfall in the northernmost area of Norway, group I strains, in contrast to group III strains, did not regrow at all. These results suggest that group III strains are more resistant than group I strains to rapid freezing Group III strains are well adapted to climatic conditions in northernmost Norway Freezing points of myceha of group III strains were determined by calonmetenc analysis Mycelia of group III strains froze at temperatures higher than -10℃ Therefore, the freezing resistance of group III strains might be based on extracellular ice formation. In the cytosol of group III, a 30 kDa protein that strongly immunoreacted with the anti-antifreeze protein (AFP) I antibody was found, and the 30 kDa protein level in group III strains was higher than that in group I strains. These results suggest that the difference in the distribution pattern of both groups can be ascribed, at least partially, to the difference in freezing resistance of myceha.}, pages = {112--118}, title = {FREEZING RESISTANCE AMONG ISOLATES OF A PSYCHROPHILIC FUNGUS, TYPHULA ISHIKARIENSIS, FROM NORWAY (19th Symposium on Polar Biology)}, volume = {11}, year = {1998} }