@article{oai:nipr.repo.nii.ac.jp:00005345, author = {SUGIZAKI, Mateus and LUCCHIARI, Pedro Helio and MALUCELLI, Maria Ivette Carboni and BACILA, Metry}, journal = {Proceedings of the NIPR Symposium on Polar Biology}, month = {Feb}, note = {P(論文), Research has been carried out on the effect of temperature on the physiological properties of mitochondria isolated from liver, cardiac muscle and encephalon of the Antarctic fish Notothenia neglecta. Mitochondria were obtained in a medium containing 0.25 M mannitol, 0.075 M sucrose, 10^<-3> M EDTA, and 10^<-2> M TRIS, in 10^<-3> M phosphate buffer, pH 7.4. All assays were carried out by means of a polarograph assembled with an oxygen electrode possessing a temperature sensor. Respiration rates, oxidative phosphorylation and respiratory control values were assayed in regard to the effect of temperature. All preparations of liver, cardiac muscle and encephalon mitochondria displayed a very efficient respiration mechanism at 1℃. No general pattern for the influence of temperature increase has been found in those preparations. On the other hand, encephalon mitochondria displayed an efficient system for ATP generation by oxidative phosphorylation, but sensitive to temperature increase.}, pages = {145--152}, title = {RESPIRATION AND OXIDATIVE PHOSPHORYLATION OF MITOCHONDRIA FROM TISSUES AND ORGANS OF ANTARCTIC FISH (18th Symposium on Polar Biology)}, volume = {10}, year = {1997} }