{"created":"2023-07-25T11:06:43.341583+00:00","id":10211,"links":{},"metadata":{"_buckets":{"deposit":"5768ca72-2a27-4d56-b78b-6b1e98f7adde"},"_deposit":{"created_by":17,"id":"10211","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"10211"},"status":"published"},"_oai":{"id":"oai:nipr.repo.nii.ac.jp:00010211","sets":[]},"author_link":["476","19576","19575","19580","19583","19581","19577","19574","19578","19579"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"51","bibliographicPageStart":"41","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Polar science"},{"bibliographic_title":"Polar science","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Viable bacterial communities on the surface of Gulkana Glacier (Alaska) were investigated using a cultivation method. Viable bacteria were isolated using R2A, diluted-R2A (DR2A), LB, diluted-LB (DLB), and xylose agar at 4, 15 and 25 °C. The highest number of colony-forming units (CFU) was observed on DR2A agar plates at 4 °C, ranging from 10^4-10^5 CFU mL^<-1>. A collection of 234 morphologically distinct isolates was obtained in total. The glacial snow and ice sample was dominated by Betaproteobacteria (97 isolates) and Gammaproteobacteria (87 isolates). The bacterial communities were examined by amplifying 16S rRNA genes from the isolates, and 34 phylotypes with >99% similarities were obtained. Of these phylotypes, 26 (76.5%) were similar to those phylotypes found in bacteria that were previously recorded from cold environments. Five of the phylotypes appeared in the clone library of a previous independent cultivation study, corresponding with 6.5% in the clone library. Our results suggest that cold environments harbor common phylotypes of culturable bacteria, which could possibly lead to a better understanding of bacterial diversity on glaciers in combination with molecular studies.","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"アラスカ・グルカナ氷河におけるバクテリア群集を培養法によって解析した．氷河表面の雪氷試料を高度別に採取し，様々な培地と温度条件で培養した．その結果，50倍希釈R2A培地を用いて，4℃で培養したときに，最大コロニー数（104-105 CFU mL-1）を示し，グルカナ氷河には，低温・貧栄養環境に適応したバクテリアが多く生息していると考えられた．培養によって得られた234株について，16S rRNA遺伝子による系統解析を行った結果，β-およびγ-Proteobacteriaに属する株が優占していることが分かった．また，培養株全体では34の系統群を示し，このうち26群（76.5％）は寒冷環境からの報告例があることから，寒冷な環境に共通した種がグルカナ氷河にも存在することが示唆された．","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立極地研究所"}]},"item_10001_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"110008426667","subitem_relation_type_select":"NAID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.polar.2010.12.002"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1016/j.polar.2010.12.002","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12240481","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"18739652","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Segawa, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimura, Yoshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Kenichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanda, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kohshima, Shiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Segawa, Takahiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshimura, Yoshitaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Kenichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kanda, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kohshima, Shiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cold environment","subitem_subject_scheme":"Other"},{"subitem_subject":"Glacier","subitem_subject_scheme":"Other"},{"subitem_subject":"Psychrophile","subitem_subject_scheme":"Other"},{"subitem_subject":"Microbial ecology","subitem_subject_scheme":"Other"},{"subitem_subject":"Cultivation","subitem_subject_scheme":"Other"},{"subitem_subject":"Phylogenetic study","subitem_subject_scheme":"Other"},{"subitem_subject":"Cold environment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glacier","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Psychrophile","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microbial ecology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cultivation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phylogenetic study","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Community structure of culturable bacteria on surface of Gulkana Glacier, Alaska","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Community structure of culturable bacteria on surface of Gulkana Glacier, Alaska"},{"subitem_title":"Community structure of culturable bacteria on surface of Gulkana Glacier, Alaska","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"17","path":["1350"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-16"},"publish_date":"2014-10-16","publish_status":"0","recid":"10211","relation_version_is_last":true,"title":["Community structure of culturable bacteria on surface of Gulkana Glacier, Alaska"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-07-25T16:20:50.802323+00:00"}