@article{oai:nipr.repo.nii.ac.jp:00010184,
 author = {N., Kurosawa and S., Sato and Y., Kawarabayasi and S., Imura and T., Naganuma and N., Kurosawa and S., Sato and Y., Kawarabayasi and S., Imura and T., Naganuma},
 issue = {2},
 journal = {Polar science, Polar science},
 month = {Aug},
 note = {Prokaryotic community structures in the anoxic sediment of the Antarctic meromictic Lake Nurume-Ike were revealed by sequence analysis of 16S rRNA gene clones. The archaeal clones obtained (205 total) consisted of only three phylotypes, and were dominantly affiliated with uncultured euryarchaeotes. Specifically, 93% of the clones were identified as marine benthic group-D archaeal phylotype. In contrast to the limited archaeal diversity, 53 phylotypes were detected within 312 bacterial clones. Major bacterial phylotypes were affiliated with α-Proteobacteria (20% of clones), d-Proteobacteria (9%), Planctmycetales (7%), and Cyanobacteria (7%). A small numbers of clones belonging to γ-Proteobacteria, Actinobacteria, Spirochaetes, Flavobacteria, and Verrucomicrobia were also found. A total of 53% of the bacterial clones, consisting of 13 phylotypes, could not be classified into any known group. These results indicated that the bacterial community of Lake Nurume-Ike sediment consisted of numerous phylogenetic groups and had a diversity comparable to the diversity of other Antarctic lakes communities previously reported. Interestingly, however, there were very few phylotypes shared between the communities of lakes Nurume-Ike and five other lakes located in the Vestfold Hills area. This is the first comprehensive study to analyze more than 500 16S rDNA clones for microbial community analysis of an Antarctic lake sediment sample, and the results significantly expand current views of bacterial diversity in Antarctic lakes., 南極Langhovde地区に存在する部分循環湖、ぬるめ池の底泥におけるアーキアとバクテリアの群集構造を、16S rDNAクローン解析法により調べた。アーキア由来の205クローンは、わずか3つの系統（phylotype）から構成され、そのうちのひとつ、Marine benthic group-Dと呼ばれるクローンだけで、アーキア全体の93%を占めていた。一方バクテリア由来の312クローンは、Proteobacteria、Planctmycetales、Cyanobacteria、Actinobacteriaなどから構成される53系統に分類され、非常に多様性に富んでいる事がわかった。また、約半数のバクテリアのクローンは、既知種との相同性が非常に低く、新属新種に由来する未培養クローンである事がわかった。},
 pages = {421--429},
 title = {Archaeal and bacterial community structures in the anoxic sediment of Antarctic meromictic lake Nurume-Ike},
 volume = {4},
 year = {2010}
}