@article{oai:nipr.repo.nii.ac.jp:00010180,
 author = {A.-C., Lautredou and C., Bonillo and G., Denys and C., Cruaud and C., Ozouf-Costaz and G., Lecointre and A., Dettai and A.-C., Lautredou and C., Bonillo and G., Denys and C., Cruaud and C., Ozouf-Costaz and G., Lecointre and A., Dettai},
 issue = {2},
 journal = {Polar science, Polar science},
 month = {Aug},
 note = {The Trematominae are a particularly interesting subfamily within the antarctic suborder Notothenioidei (Teleostei). The 14 closely related species occupy a large range of ecological of niches, extremely useful for evolutionary and biogeography studies in the Antarctic Ocean. But some Trematomus species can be difficult to identify by using morphological criteria, specially young stages and damaged specimens. Molecular identification would therefore be highly useful, however the suitability of the cytochrome oxidase I gene in a barcoding approach needs to be assessed. We evaluated species delineation within the genus Trematomus comparing morphological identification, nuclear markers (the rhodopsin retrogene and a new nuclear marker pkd1: polycystic kidney disease 1) and COI. We show that Trematomus vicarius is not distinguishable from Trematomus bernacchii with the molecular markers used, and neither is Trematomus loennbergii from Trematomus lepidorhinus. We suggest that until this is investigated further, studies including these species list them as T. loennbergii/T. lepidorhinus group, and keep voucher samples and specimens. Generally, COI gives a congruent result with the rhodopsin retrogene, and except for the previously cited species pairs, COI barcoding is efficient for identification in this group. Moreover pkd1 might not be suitable for a phylogenetic study at this scale for this group., Trematomus属はノトセニア亜目に所属し、南極海に生息する14種類はその進化や生物地理学分野の研究に用いられている。しかし若いステージや破損した試料では形態的な種判別が困難な場合がある。そのため分子生物的手法による種同定の有用性を検証すべく、形態観察、核マーカーおよびCOI領域を用いた種判別の比較および評価を行なった。分子マーカーを用いた分析では、T. vicariusとT. bernacchii、またT. loennbergiiとT. lepidorhinusの区別が困難であった。この２ペアに関しては今後さらなる調査を実施する必要がある。上記２ペアを除いたTrematomus属の種判別にはCOI領域が最も効率的であった。},
 pages = {333--352},
 title = {Molecular taxonomy and identification within the Antarctic genus Trematomus (Notothenioidei, Teleostei): How valuable is barcoding with COI?},
 volume = {4},
 year = {2010}
}