@article{oai:nipr.repo.nii.ac.jp:00010173,
 author = {C.P., Foong and C.M., Wong Vui Ling and M., Gonzalez and C.P., Foong and C.M., Wong Vui Ling and M., Gonzalez},
 issue = {2},
 journal = {Polar science, Polar science},
 month = {Aug},
 note = {There is little information on the bacterial diversity of the Fildes Peninsula, King George Island. Hence, this study was conducted to determine the bacterial population of sediments and soils from the lakes, river, glacier and an abandoned oil tank area in the Fildes Peninsula, using a metagenomic approach. DNA was extracted from the sediment and soil samples, and analyzed using the 16S rDNA polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). A total of 299 DNA fragments resolved using the DGGE were sequenced. The results of the analysis provided an overview of the predominant groups of bacteria and the diversity of the bacterial communities. The most abundant phyla of bacteria in Fildes Peninsula were Bacteroidetes, Proteobacteria, Acidobacteria, Gemmatimonadetes, Nitrospira, Firmicutes, Actinobacteria, Chloroflexi, Cyanobacteria, Spirochaetes, Deinococcus-Thermus, WS3 and BRC1. All of the sediment samples from the lakes had different representatives of dominant bacterial species. Interestingly, 15% of the operational taxonomic units (OTUs) did not group into any of the existing phyla in the Ribosomal Database Project (RDP). One of the OTUs had a similarity of <0.90 when compared to the GenBank sequences and probably was a novel bacterium specific to that location. The majority of the bacterial 16S rDNA sequences were found to be closely related to those found elsewhere., 南極キングジョージ島Fildes半島の湖沼、河川、氷河の堆積物試料におけるバクテリア群集を決定した。16SrDNAのPCR-DGGE法によって分析され、計299のDNA断片の配列をもとに系統樹が描かれた結果、卓越する細菌類のグループやサンプル間の細菌群集の多様性の概略が明らかになった。湖沼からの10サンプルは、異なる優占細菌種を持っていた。興味深いことに、OTUの15%は、データベースにある既知の系統とは異なっていた。あるOUTは、GenBankにあるシーケンスと90%以下の類似度しかなく、おそらく新種であると考えられる。一方、大部分の細菌の16SrDNA配列は、地球上のあらゆる場所に見つかる細菌のそれときわめて近いことが分かった。},
 pages = {263--273},
 title = {Metagenomic analyses of the dominant bacterial community in the Fildes Peninsula,King George Island (South Shetland Islands)},
 volume = {4},
 year = {2010}
}