Item type |
紀要論文(ELS) / Departmental Bulletin Paper(1) |
公開日 |
1998-02-01 |
タイトル |
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タイトル |
RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF 16S rRNA GENE OF THE FAMILY VIBRIONACEAE ISOLATED FROM COLD MARINE ENVIRONMENTS (19th Symposium on Polar Biology) |
言語 |
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言語 |
eng |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
marine bacteria |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Vibnonaceae |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
16S rRNA |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
psychrophile |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
PCR-RFLP |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
ID登録 |
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ID登録 |
10.15094/00005360 |
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ID登録タイプ |
JaLC |
ページ属性 |
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内容記述タイプ |
Other |
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内容記述 |
P(論文) |
記事種別(英) |
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en |
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Proceeding |
論文名よみ |
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その他のタイトル |
RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF 16S rRNA GENE OF THE FAMILY VIBRIONACEAE ISOLATED FROM COLD MARINE ENVIRONMENTS (19th Symposium on Polar Biology) |
著者名よみ |
ウラカワ, ヒデトシ
キタ-ツカモト, クミコ
オオワダ, コウイチ
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著者名(英) |
URAKAWA, Hidetoshi
KITA-TSUKAMOTO, Kumiko
OHWADA, Kouichi
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著者所属(英) |
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en |
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Ocean Research Institute, University of Tokyo |
著者所属(英) |
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en |
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Ocean Research Institute, University of Tokyo |
著者所属(英) |
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en |
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Ocean Research Institute, University of Tokyo |
抄録(英) |
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内容記述タイプ |
Other |
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内容記述 |
The present study was carried out for grouping and characterization of 60 natural isolates of psychrophihc and psychrotrophic vibrios isolated from deep-sea areas of the Western Pacific and Otsuchi Bay, where are located in northern Japan, using PCR-RFLP analysis of 16S rRNA genes. The 16S rRNA genes, about 1500bp long, were amplified by PCR and used to determine the genotypes and phylogenetic affiliation of 60 natural isolates Four tetramenc restriction enzymes (Hhal, Ddel, Rsal and 5aw3AI) were used for restriction fragment length polymorphism (RFLP) analysis. The 21 type and reference of the family Vibnonaceae were included in the analysis. A total of 81 strains were separated into 24 RFLP patterns Five groups were obtained from the genetic distance tree and results showed significant differences in the genotype composition between the isolates from the deep-sea and Otsuchi Bay. We observed two large phylogenetic groups in psychrophihc and psychrotrophic vibrios. One was Group 5 (the Vibrio marinus group) which was distributed only in the depths of the Western Pacific The other was Group 3 (the OC02 group) which was distributed in Otsuchi Bay. |
雑誌書誌ID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10819561 |
書誌情報 |
Proceedings of the NIPR Symposium on Polar Biology
巻 11,
p. 8-21,
発行日 1998-02
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出版者 |
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出版者 |
National Institute of Polar Research |