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Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy
https://nipr.repo.nii.ac.jp/records/10218
https://nipr.repo.nii.ac.jp/records/102182fdaeb3b-7af3-49e0-bb7d-c735e1e4e15a
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-10-16 | |||||
タイトル | ||||||
タイトル | Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Bacteria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Microdissection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Single cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 16S rRNA | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Bacteria | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Microdissection | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Single cell | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | 16S rRNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Yanagihara, Katsuhiko
× Yanagihara, Katsuhiko× Niki, Hironori× Baba, Tomoya× Yanagihara, Katsuhiko× Niki, Hironori× Baba, Tomoya |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Here, we describe a technique that allows the genetic linage analysis of 16S rRNA genes in bacteria observed under a microscope. The technique includes the isolation of microbial cells using a laser microdissection microscope, lysis of the cells, and amplification of the 16S rRNA genes in the isolated cells without interference by bacterial DNA contamination from the experimental environment or reagents. Using this technique, we successfully determined 15 16S rRNA gene sequences in cells isolated from an Antarctic iceberg. These sequences showed 94-100% identity to their closest strains, which included bacteria that occur in aqueous, marine, and soil environments. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 我々はこの論文において、顕微鏡下で観察した細菌から16S rRNA遺伝子の遺伝系統解析をする技術を記述する。これには、レーザーマイクロダイセクション顕微鏡を用いた微生物細胞の単離と、その細胞の溶解、実験環境や試薬を汚染している細菌のゲノムDNAの干渉を受けずに単離した細胞の16S rRNA遺伝子を増幅する方法、が含まれる。この技術を用いて、我々は南極氷山試料から単離した15細胞について、その16S rRNA遺伝子の配列を決定することができた。それらの配列は、水、海洋、土壌環境でこれまでに発見されていた細菌株の16S rRNA遺伝子と比較し、94-100%の一致率を示した。 | |||||
書誌情報 |
Polar science en : Polar science 巻 5, 号 3, p. 375-382, 発行日 2011-09 |
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出版者 | ||||||
出版者 | 国立極地研究所 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18739652 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12240481 | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 110008667900 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1016/j.polar.2011.06.001 | |||||
関連名称 | 10.1016/j.polar.2011.06.001 |