WEKO3
アイテム
{"_buckets": {"deposit": "56c36048-e5cf-466a-b0fd-67f8628ac0e1"}, "_deposit": {"created_by": 17, "id": "10168", "owners": [17], "pid": {"revision_id": 0, "type": "depid", "value": "10168"}, "status": "published"}, "_oai": {"id": "oai:nipr.repo.nii.ac.jp:00010168", "sets": []}, "author_link": ["19163", "19164", "19157", "19161", "19158", "19165", "19159", "19153", "19162", "19154", "19160", "19155", "19152", "19156"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2010-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "244", "bibliographicPageStart": "237", "bibliographicVolumeNumber": "4", "bibliographic_titles": [{"bibliographic_title": "Polar science"}, {"bibliographic_title": "Polar science", "bibliographic_titleLang": "en"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The protozoan ciliate Euplotes nobilii found in Antarctic and Arctic coastal waters relies on secretion of water-soluble cell type-specific signal proteins (pheromones) to regulate its vegetative growth and sexual mating. For three of these psychrophilic pheromones we previously determined the three-dimensional structures by nuclear magnetic resonance (NMR) spectroscopy with protein solutions purified from the natural sources, which led to evidence that their adaptation to cold is primarily achieved by increased flexibility through an extension of regions free of regular secondary structures, and by increased exposure of negative charges on the protein surface. Then we cloned the coding genes of these E. nobilii pheromones from the transcriptionally active cell somatic nucleus (macronucleus) and characterized the full-length sequences. These sequences all contain an open reading frame of 252–285 nucleotides, which is specific for a cytoplasmic pheromone precursor that requires two proteolytic cleavages to remove a signal peptide and a pro segment before release of the mature protein into the extracellular environment. The 5′ and 3′ non-coding regions are two- to three-fold longer than the coding region and appear to be tightly conserved, probably in relation to the inclusion of intron sequences destined to be alternatively removed to play key regulatory roles in the mechanism of the pheromone gene expression.", "subitem_description_type": "Abstract"}]}, "item_10001_description_6": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "南北両極の沿岸水域に見られるEuplotes nobiliiは、その栄養成長と交配の制御を、水溶性の特別な信号タンパク質(フェロモン)の分泌に依存している。我々は、転写活性の高い大核からこれらのフェロモンをコードする遺伝子をクローニングし、遺伝子配列の全長を明らかにした。これらの配列はすべて、252から285塩基対の翻訳領域を持ち、成熟したタンパク質として細胞外環境に放出される前に一部を切断されることが必要なフェロモン前駆体である。5’、3’両末端の非翻訳領域は、翻訳領域の2,3倍の長さを持ち、保存性が高い。この部分はおそらく、フェロモンの遺伝子発現のメカニズムにおいて、活性を獲得するために取り除かれる筈だったイントロン配列を取り込んだものと思われる。", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "国立極地研究所"}]}, "item_10001_relation_12": {"attribute_name": "論文ID(NAID)", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "110007702215", "subitem_relation_type_select": "NAID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.polar.2010.03.007"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1016/j.polar.2010.03.007", "subitem_relation_type_select": "DOI"}}]}, "item_10001_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12240481", "subitem_source_identifier_type": "NCID"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "18739652", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "A., Vallesi"}], "nameIdentifiers": [{"nameIdentifier": "19152", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "C., Alimenti"}], "nameIdentifiers": [{"nameIdentifier": "19153", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "G., Di Giuseppe"}], "nameIdentifiers": [{"nameIdentifier": "19154", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "F., Dini"}], "nameIdentifiers": [{"nameIdentifier": "19155", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "B., Pedrini"}], "nameIdentifiers": [{"nameIdentifier": "19156", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "K., Wuthrich"}], "nameIdentifiers": [{"nameIdentifier": "19157", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "P., Luporini"}], "nameIdentifiers": [{"nameIdentifier": "19158", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "A., Vallesi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19159", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "C., Alimenti", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19160", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "G., Di Giuseppe", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19161", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "F., Dini", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19162", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "B., Pedrini", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19163", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "K., Wuthrich", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19164", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "P., Luporini", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "19165", "nameIdentifierScheme": "WEKO"}]}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Polar microorganisms", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Macronuclear gene-sized DNA molecules", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cold-adapted signal proteins", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ciliate mating-type systems", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Polar microorganisms", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Macronuclear gene-sized DNA molecules", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cold-adapted signal proteins", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ciliate mating-type systems", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures"}, {"subitem_title": "The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "17", "path": ["1355"], "permalink_uri": "https://nipr.repo.nii.ac.jp/records/10168", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-10-16"}, "publish_date": "2014-10-16", "publish_status": "0", "recid": "10168", "relation": {}, "relation_version_is_last": true, "title": ["The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures"], "weko_shared_id": -1}
The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures
https://nipr.repo.nii.ac.jp/records/10168
https://nipr.repo.nii.ac.jp/records/101689f2ac700-7814-4fc2-9052-5acaa3af867f
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2014-10-16 | |||||
タイトル | ||||||
タイトル | The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The water-born protein pheromones of the polar protozoan ciliate, Euplotes nobilii : Coding genes and molecular structures | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Polar microorganisms | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Macronuclear gene-sized DNA molecules | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cold-adapted signal proteins | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ciliate mating-type systems | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Polar microorganisms | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Macronuclear gene-sized DNA molecules | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cold-adapted signal proteins | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Ciliate mating-type systems | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
A., Vallesi
× A., Vallesi× C., Alimenti× G., Di Giuseppe× F., Dini× B., Pedrini× K., Wuthrich× P., Luporini× A., Vallesi× C., Alimenti× G., Di Giuseppe× F., Dini× B., Pedrini× K., Wuthrich× P., Luporini |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The protozoan ciliate Euplotes nobilii found in Antarctic and Arctic coastal waters relies on secretion of water-soluble cell type-specific signal proteins (pheromones) to regulate its vegetative growth and sexual mating. For three of these psychrophilic pheromones we previously determined the three-dimensional structures by nuclear magnetic resonance (NMR) spectroscopy with protein solutions purified from the natural sources, which led to evidence that their adaptation to cold is primarily achieved by increased flexibility through an extension of regions free of regular secondary structures, and by increased exposure of negative charges on the protein surface. Then we cloned the coding genes of these E. nobilii pheromones from the transcriptionally active cell somatic nucleus (macronucleus) and characterized the full-length sequences. These sequences all contain an open reading frame of 252–285 nucleotides, which is specific for a cytoplasmic pheromone precursor that requires two proteolytic cleavages to remove a signal peptide and a pro segment before release of the mature protein into the extracellular environment. The 5′ and 3′ non-coding regions are two- to three-fold longer than the coding region and appear to be tightly conserved, probably in relation to the inclusion of intron sequences destined to be alternatively removed to play key regulatory roles in the mechanism of the pheromone gene expression. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 南北両極の沿岸水域に見られるEuplotes nobiliiは、その栄養成長と交配の制御を、水溶性の特別な信号タンパク質(フェロモン)の分泌に依存している。我々は、転写活性の高い大核からこれらのフェロモンをコードする遺伝子をクローニングし、遺伝子配列の全長を明らかにした。これらの配列はすべて、252から285塩基対の翻訳領域を持ち、成熟したタンパク質として細胞外環境に放出される前に一部を切断されることが必要なフェロモン前駆体である。5’、3’両末端の非翻訳領域は、翻訳領域の2,3倍の長さを持ち、保存性が高い。この部分はおそらく、フェロモンの遺伝子発現のメカニズムにおいて、活性を獲得するために取り除かれる筈だったイントロン配列を取り込んだものと思われる。 | |||||
書誌情報 |
Polar science en : Polar science 巻 4, 号 2, p. 237-244, 発行日 2010-08 |
|||||
出版者 | ||||||
出版者 | 国立極地研究所 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18739652 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12240481 | |||||
論文ID(NAID) | ||||||
識別子タイプ | NAID | |||||
関連識別子 | 110007702215 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1016/j.polar.2010.03.007 | |||||
関連名称 | 10.1016/j.polar.2010.03.007 |